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World Health Organization International Standard To ...

Sep 01, 2015 · In section 2.6.7 of the Ph. Eur., the minimal sensitivity of an NAT is defined to correspond to 10 CFU/ml for replacement of the culture-based assays or to 100 CFU/ml for replacement of the indicator cell culture method , whereas in the corresponding chapter (chapter 63) of the United States Pharmacopeia, the sensitivity required for a ...

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3.2 TEST FOR STERILITY

Japanese Pharmacopoeia (JP) and United States Pharmacopeia (USP). This internationally harmonized test replaces the current method 3.2.1 Test for sterility of non-injectable preparations and 3.2.2 Sterility testing of antibiotics. As a consequence, all references to 3.2.1 and 3.2.2 in …

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Conserving Supply of Personal Protective Equipment—A Call ...

The editors of JAMA recognize the challenges, concerns, and frustration about the shortage of personal protective equipment (PPE) that is affecting the care of patients and safety of health care workers in the US and around the world. We seek creative immediate solutions for how to maximize the use of PPE, to conserve the supply of PPE, and to identify new sources of PPE.

Author: Howard Bauchner, Phil B. Fontanarosa, Edward H. Livingston

Review article: the global emergence of Helicobacter ...

Dec 23, 2015 · Current treatment of H. pylori in the United States is empiric despite relatively high failure rates in more than 20% of cases.18 In the early 1990s the eradication rate of H. pylori was greater than 80%.19, 20, 21 However, the antimicrobial eradication rates are decreasing – as low as 60% in some countries – and are inversely correlated with antibiotic resistance rates reported worldwide.19, …

Cited by: 293

Validation of a real-time PCR assay for the molecular ...

Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis Mariana L. Sales , 1 Antônio Augusto Fonseca, Júnior , 1 Lívia Orzil , 1 Andrea Padilha Alencar , 1 Marcio Roberto Silva , 2 Marina Azevedo Issa , 1 Paulo Martins Soares Filho , 1 Andrey Pereira Lage , 3 and Marcos Bryan Heinemann 3

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The Challenge of Prion Sterilization

The problem with prions is that they are incredibly resistant to most routine methods of decontamination and sterilization. In fact, experts featured in the above Oxford Journal post believe there is no method of prion decontamination or sterilization that has proven 100% effective.

Search for QIAcube Standard Protocols - QIAGEN

You can search for protocols and protocol sheets by selecting an application, kit, or starting material. For a more refined search, select more than one parameter. A link to the relevant protocol sheet is provided in the detailed information about the protocol. Find out how to transfer protocols to your QIAcube.

Rapid and visual detection of Salmonella in meat using ...

It causes bacterial gastroenteritis and is responsible for approximately 30% of foodborne outbreaks in the United States (Crump & Mintz, 2010) and 23% in European Union . Globally, non-typhoidal Salmonella is accountable for 153 million cases (approx) of gastroenteritis and 57,000 deaths each year ( Hunter & Francois Watkins, 2019 ).

Author: G. Bhuvana Priya, Ravi Kant Agrawal, A. Arun Prince Milton, Madhu Mishra, S.K. Mendiratta, Ashish Lu...

Screening of biomarkers of drug resistance or virulence in ...

Dec 12, 2019 · Rapid identification and characterisation of drug-resistant bacterial pathogens have an important role in diagnostic and antimicrobial stewardship. Response time in …

Author: Samantha Flores-Treviño, Elvira Garza-González, Soraya Mendoza-Olazarán, Rayo Morfín-Otero, Adrián C...

The Concept of an Ideal Antibiotic: Implications for Drug ...

Mar 03, 2019 · Based on data from the United States, more than 80% of drugs in current use are orally administered; therefore this route should be primarily targeted [102,103]. This is especially true for the treatment of infectious diseases, where intravenous (IV) administration should only be used, if it is justified by the medical condition of the patient.

Cited by: 32

Meiotic DNA break formation requires the unsynapsed ...

Introduction. Generation of haploid gametes requires segregation of homologous chromosomes (homologues) during the first meiotic division. In most taxa including mammals, each pair of homologues must engage and become physically linked via at least one crossover during the first prophase to achieve high fidelity segregation 1,2.Inter-homologue crossovers are formed by homologous recombination ...

Cited by: 83

Isolation of Drug-Resistant Gallibacterium anatis from ...

Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 ...

Frontiers | Modeling Metabolic Interactions in a ...

Dec 14, 2017 · The gut microbiome is a complex microbial community that has a significant influence on the host. Microbial interactions in the gut are mediated by dietary substrates, especially complex polysaccharides. In this environment, breakdown products from larger carbohydrates and short chain fatty acids are commonly shared among gut microbes.

Cited by: 9

PANDEMIC PLANNING - CDC

Background

Effects of nutritional and ambient oxygen condition on ...

Feb 03, 2017 · First, planktonic bacteria (OD 660 0.5) and pellicle bacteria were prepared in 96-well plates in 7H9Eut under normoxic and 5% O 2 condition, …

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Gut Microbiota-Based Pharmacokinetics and the ...

Mar 20, 2019 · Paeoniflorin, the main component of Xiaoyao Wan, presents low oral bioavailability and unclear antidepressant mechanism. To elucidate the potential reasons for the low bioavailability of paeoniflorin and explore its antidepressant mechanism from the perspective of the gut microbiota, here, a chronic unpredictable depression model and forced swimming test were firstly performed to examine …

Cited by: 2

Cytokine Profiles of Severe Influenza Virus-Related ...

Nov 06, 2017 · Introduction. Influenza virus is one of the leading causes of hospitalizations and deaths in children ().Influenza-related lower respiratory tract infection that progresses to acute lung injury (ALI) with profound hypoxia and respiratory failure, or to systemic inflammation leading to circulatory collapse requiring vasopressors (i.e., septic shock), is often deadly.

Cited by: 5

Recombinant human soluble thrombomodulin is associated ...

Multiple organ dysfunction induced by sepsis often involves kidney injury. Extracellular histones released in response to damage-associated molecular patterns are known to facilitate sepsis-induced organ dysfunction. Recombinant human soluble thrombomodulin (rhTM) and its lectin-like domain (D1) exert anti-inflammatory effects and neutralize damage-associated molecular patterns. However, the ...

Author: Masayuki Akatsuka, Yoshiki Masuda, Hiroomi Tatsumi, Michiaki Yamakage

Clinical Evaluation of a Loop-Mediated Isothermal ...

Apr 08, 2015 · Recently, polymerase chain reaction (PCR)-based diagnostic methods of detecting Nm have been considered the gold standard because of their superior sensitivity and specificity compared with culture. In this study, we developed a loop-mediated isothermal amplification (LAMP) method and evaluated its ability to detect Nm in cerebrospinal fluid (CSF).

Cited by: 32

Validation of a real-time PCR assay for the molecular ...

Introduction. Tuberculosis (TB) is one of the oldest and most serious known diseases. Studies using DNA isolated from cattle bones found in North America, which were dated to 17,000 BC, showed the presence of Mycobacterium tuberculosis (Rothschild et al., 2001).During the Industrial Revolution, numerous cases of tuberculosis emerged due to the agglomeration of workers (Costa, 1985).

Frontiers | Insight Into the Microbial Co-occurrence and ...

Aug 13, 2019 · The microbial community within the CG tumor has not been widely elucidated and it is not known if certain members of this microbial community promote or inhibit CG. This study investigated the microbiotas of grapevine CG tumor tissues from seven infected vineyards located in Hungary, Japan, Tunisia, and the United States.

Seasonality of antimicrobial resistance rates in ...

pneumoniae to penicillins and cephalosporins [4, 10–12], whereas AMR rates to macrolides showed no seasonal variation . A study in the United States showed that the rates of penicillin-resistant S. pneumoniae were higher in spring than in winter , while other studies in Spain showed higher resistance rates in both summer and winter [3, 13].

Named Entity Recognition for Bacterial Type IV Secretion ...

Introduction. Named entity recognition (NER) research has focused on recognition of classes such as genes, proteins, and diseases. We explored recognition of less-studied classes of entities, such as cellular components and biological processes, to support enhanced access to the literature for users of the Pathosystems Resource Integration Center (PATRIC, patricbrc).

Development, validation, and responsiveness of a novel ...

Validation and responsiveness assessment of DAIBD. A: Distribution of the combined weighting and validation cohort (n = 160) stratified by the physician's global assessment of disease activity. B: Delta DAIBD (the difference in the score between baseline and follow-up visits) stratified by physician's global assessment of change (DAIBD ...

Epidemiology, Diagnosis, and Antimicrobial Treatment of ...

EPIDEMIOLOGY AND VACCINATION. Studies of the incidence of bacterial meningitis performed in the United States during the 1950s, 1960s, and 1970s found significant attack rates for the common meningeal pathogens at that time (Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae), although these case-finding efforts were performed with relatively small populations.

Activity of Novel Synthetic Peptides against Candida ...

May 12, 2015 · Candida spp. are the most common causes of fungal infections worldwide. Among the Candida species, Candida albicans remains the predominant species that causes invasive candidiasis in …

PANDEMIC PLANNING - CDC

This document recommends practices for extended use and limited reuse of NIOSH-certified N95 filtering facepiece respirators (commonly called “N95 respirators”). The recommendations are intended for use by professionals who manage respiratory protection programs in healthcare institutions to protect health care workers from job-related risks of exposure to infectious respiratory illnesses.

Clostridium difficile infection: new developments in ...

The community CDI rates in the United States have been reported as 7.7 cases per 100,000 person years, of which 35% received no antibiotics within 42 days of C. difficile detection 21.

Strategies in the design of antiviral drugs | Nature ...

Greater understanding of viral life cycles has resulted in the discovery and validation of several targets for therapeutic intervention, and an increase in the number of antiviral drugs from 5 in ...

The salivary microbiome is consistent between subjects and ...

We chose to perform a 72 hour follow-up because the average hospital stay in the United States is approximately 4.5 days 52. Lastly, we included patients with a variety of diagnoses in our short ...

Evaluating the efficacy of cloth facemasks in reducing ...

Evaluating the efficacy of cloth facemasks in reducing particulate matter exposure Article in Journal of Exposure Science and Environmental Epidemiology 27(3) · August 2016 with 1,007 Reads

VITEK MS | bioMérieux Clinical Diagnostics

VITEK ® MS v3 / KB v3.2.0 is pending 510(k) clearance and is not available for commercial sale in the United States. Optimized workflow and Seamless ID/AST integration. VITEK ® MS is designed specifically for optimized workflow in the microbiology laboratory.

From infection to immunotherapy: host immune responses …

Sep 25, 2013 · Bladder cancer is one of the most prevalent cancers, with reported rates of >72,500 people per year in the United States and >100,000 individuals in Western Europe. 153, 154 It is noteworthy that ...

VWR, Part of Avantor - Chemicals and Laboratory Scientific ...

Avantor ® is a trusted global partner to customers and suppliers in the life science, advanced technology and applied materials industries. We operate in more than 30 countries and deliver an extensive portfolio of mission critical products, services and solutions. As our channel brand, VWR offers an integrated, seamless purchasing experience that is optimized for the way our customers do ...

Influence of Nebulizer Type With Different Pediatric ...

BACKGROUND: The performance of nebulizers varies with the design type as well as the breathing patterns of various age groups. The present study quantified aerosol delivery using spontaneously breathing parameters of a small child (2–4 years) by a lung simulator to determine the influence of nebulizer type, actuation mechanisms, and pediatric aerosol masks. METHODS: Three types of …

Antibiotic exposure perturbs the gut microbiota and ...

Antibiotic treatment of bee colonies has been widely used for over 50 y in the United States to prevent a bacterial disease of bee larvae called foulbrood (Paenibacillus larvae) [23–25]. The two antibiotics most commonly used by beekeepers are tetracycline (or the related …

Clindamycin | C18H33ClN2O5S - PubChem

Clindamycin is used as an alternative agent for prevention of a-hemolytic (viridans group) streptococcal bacterial endocarditis in penicillin-allergic adults and children with certain cardiac conditions who are undergoing certain dental procedures (i.e., procedures that involve manipulation of gingival tissue, the periapical region of teeth, or perforation of oral mucosa) or certain invasive ...

Antimicrobial effect of surgical masks coated with ...

Antimicrobial effect of surgical masks coated with nanoparticles Article in Journal of Hospital Infection 62(1):58-63 · February 2006 with 2,619 Reads How we measure 'reads'

Antimicrobial Stewardship - CIDRAP

Campbell D, Bowen A, Bhatnagar A, et al. Identification and characterization of Shigella with decreased susceptibility to azithromycin in the United States, 2005 to 2014. J Glob Antimicrob Resist 2019 (published online Dec 19) Cannas A, Butera O, Gualano G, et al. Multidrug-resistant tuberculosis in a referral center In Rome: 2011–2016.

Metabolic profiling strategy for discovery of nutritional ...

In addition to the standard dinner, a supplementary mixed-fruit meal (apple, orange, grapes, and grapefruit) was introduced on the evening of day 2. Urine was collected 4 times/d (first morning urine, before lunch, before dinner, and at bed time), from the morning of day 1 until the evening of day 3.

HA-Tag Antibody 66006-1-Ig | Proteintech

HA-Tag antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), CoImmunoprecipitation (IP), Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with recombinant protein samples. Cat.No. 66006-1-Ig.

Fate of Antibiotic Resistant Bacteria and Genes during ...

This study investigated fates of nine antibiotic-resistant bacteria as well as two series of antibiotic resistance genes in wastewater treated by various doses of chlorine (0, 15, 30, 60, 150 and 300 mg Cl2 min/L). The results indicated that chlorination was effective in inactivating antibiotic-resistant bacteria. Most bacteria were inactivated completely at the lowest dose (15 mg Cl2 min/L ...

TOXICOLOGICAL PROFILE FOR BROMOFORM AND …

Bromoform. No studies were located regarding cardiovascular effects in humans after oral exposure to bromoform. Histological examination of rats and mice exposed to up to 100–200 mg/kg (5 days/week) bromoform by gavage for up to 2 years revealed no evidence of …

DDDDK Tag Antibody (66008-2-Ig) | Proteintech

DDDDK Tag Antibody (Equivalent To FLAG® Antibodies) mouse monoclonal antibody by Proteintech. Validated in WB, IP and IF to detect recombinant protein in human, mouse, pig and rat tissues. Referenced in 25 publications, visit the Proteintech website to view validation and citation data. Order now for next day delivery.

Use of the Soft-agar Overlay Technique to Screen for ...

Recently, there has been significant interest in deepening our understanding of microbial ecology (particularly the microbiomes of various environments), as well as new antibacterial compounds to use in combating antibiotic resistant pathogens 1,2.An interconnecting theme between these interests is understanding antagonistic interactions among bacterial strains in their natural environment.

CosmeticsAndToiletries - Cosmetics & Toiletries

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2:00PM Water Cooler 3/24/2020 | naked capitalism

13 days ago · We knew then that America’s arsenal of antibiotics was not sufficient to guarantee we could manage a large outbreak of drug-resistant bacteria. Since then, these bacteria have only become more widespread, more deadly, and far more difficult to treat, yet our stable of antibiotics to manage them has barely increased.” • No money in it.

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